 Improved extraction of high quality RNA from tissue samples
01 Jul 2009 Amsbio has announced a new kit for the extraction of high quality RNA from FFPE (Formalin-Fixed and Paraffin-Embedded) tissues that can be applied not only to PCR but also microarray applications. Traditional methods of formalin-fixation of tissues often result in severe RNA fragmentation, as well as in RNA-RNA, RNA-DNA and RNA-protein cross-linking, which impairs RNA solubilisation and template activity in reverse transcription and subsequent downstream assays. The new ExpressArt® FFPE RNA-ready kit is optimised for isolation of total RNA from archival, formaldehyde or FFPE tissues and results in a concentrated RNA product that is ready-to-use. Using special lysis and incubation conditions ExpressArt FFPE RNA-ready isolation and mRNA amplification technology is able to extract high quality RNA and amplify very small amounts and degrad...
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